کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2039128 1073027 2015 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A Small Molecule that Induces Intrinsic Pathway Apoptosis with Unparalleled Speed
ترجمه فارسی عنوان
یک مولکول کوچک که باعث آپوپتوز مسیر درونی خود با سرعت بی نظیر می شود
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی


• Discovery of a compound that induces unusually rapid apoptosis
• Identification of specific mitochondrial processes critical to apoptotic induction

SummaryApoptosis is generally believed to be a process that requires several hours, in contrast to non-programmed forms of cell death that can occur in minutes. Our findings challenge the time-consuming nature of apoptosis as we describe the discovery and characterization of a small molecule, named Raptinal, which initiates intrinsic pathway caspase-dependent apoptosis within minutes in multiple cell lines. Comparison to a mechanistically diverse panel of apoptotic stimuli reveals that Raptinal-induced apoptosis proceeds with unparalleled speed. The rapid phenotype enabled identification of the critical roles of mitochondrial voltage-dependent anion channel function, mitochondrial membrane potential/coupled respiration, and mitochondrial complex I, III, and IV function for apoptosis induction. Use of Raptinal in whole organisms demonstrates its utility for studying apoptosis in vivo for a variety of applications. Overall, rapid inducers of apoptosis are powerful tools that will be used in a variety of settings to generate further insight into the apoptotic machinery.

Graphical AbstractFigure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 13, Issue 9, 1 December 2015, Pages 2027–2036
نویسندگان
, , , , , , , , , , ,