کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2039182 1073032 2015 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Transcriptional Landscape of Cardiomyocyte Maturation
ترجمه فارسی عنوان
چشم انداز ترکیب بلوغ کاردیومیوسیت
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی


• Transcriptional landscape during cardiomyocyte maturation
• A GRN-based method to assess maturation status of cardiomyocytes is developed
• PSC-CMs undergo maturation but are arrested at the late embryonic stage
• Misregulated transcriptional factors may alter PSC-CM maturation in vitro

SummaryDecades of progress in developmental cardiology has advanced our understanding of the early aspects of heart development, including cardiomyocyte (CM) differentiation. However, control of the CM maturation that is subsequently required to generate adult myocytes remains elusive. Here, we analyzed over 200 microarray datasets from early embryonic to adult hearts and identified a large number of genes whose expression shifts gradually and continuously during maturation. We generated an atlas of integrated gene expression, biological pathways, transcriptional regulators, and gene regulatory networks (GRNs), which show discrete sets of key transcriptional regulators and pathways activated or suppressed during CM maturation. We developed a GRN-based program named MatStatCM that indexes CM maturation status. MatStatCM reveals that pluripotent-stem-cell-derived CMs mature early in culture but are arrested at the late embryonic stage with aberrant regulation of key transcription factors. Our study provides a foundation for understanding CM maturation.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 13, Issue 8, 24 November 2015, Pages 1705–1716
نویسندگان
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