کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2039194 1073034 2016 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Active and Inactive Enhancers Cooperate to Exert Localized and Long-Range Control of Gene Regulation
ترجمه فارسی عنوان
تقویت کننده های فعال و غیر فعال همکاری می کنند تا کنترل موضعی و بلند مدت رژیم ژنی را اعمال کنند
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی


• Synergistic contacts between the elements of a super-enhancer contribute to activity
• Igk enhancers play diverse roles in lymphocyte development
• The Igk enhancer, MiEκ, contributes to regulation of Tcrb recombination
• Enhancer sharing can involve active as well as inactive regulatory elements

SummaryV(D)J recombination relies on the presence of proximal enhancers that activate the antigen receptor (AgR) loci in a lineage- and stage-specific manner. Unexpectedly, we find that both active and inactive AgR enhancers cooperate to disseminate their effects in a localized and long-range manner. Here, we demonstrate the importance of short-range contacts between active enhancers that constitute an Igk super-enhancer in B cells. Deletion of one element reduces the interaction frequency between other enhancers in the hub, which compromises the transcriptional output of each component. Furthermore, we establish that, in T cells, long-range contact and cooperation between the inactive Igk enhancer MiEκ and the active Tcrb enhancer Eβ alters enrichment of CBFβ binding in a manner that impacts Tcrb recombination. These findings underline the complexities of enhancer regulation and point to a role for localized and long-range enhancer-sharing between active and inactive elements in lineage- and stage-specific control.

Graphical AbstractFigure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 15, Issue 10, 7 June 2016, Pages 2159–2169
نویسندگان
, , , , , , , , , , , ,