کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2039200 1073034 2016 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Complexin 3 Increases the Fidelity of Signaling in a Retinal Circuit by Regulating Exocytosis at Ribbon Synapses
ترجمه فارسی عنوان
کمپلکسین 3 با استفاده از تنظیم اگزوزتوز در سیناپس ریبن، وفاداری سیگنالینگ را در یک شبکه شبکیه افزایش می دهد
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی


• Cplx3 boosts fast phasic transmitter release while suppressing asynchronous release
• Transmission at rod bipolar cell ribbon synapses is sluggish in absence of Cplx3
• Sustained depolarization of postsynaptic interneurons degrades light-evoked signaling

SummaryComplexin (Cplx) proteins modulate the core SNARE complex to regulate exocytosis. To understand the contributions of Cplx to signaling in a well-characterized neural circuit, we investigated how Cplx3, a retina-specific paralog, shapes transmission at rod bipolar (RB)→AII amacrine cell synapses in the mouse retina. Knockout of Cplx3 strongly attenuated fast, phasic Ca2+-dependent transmission, dependent on local [Ca2+] nanodomains, but enhanced slower Ca2+-dependent transmission, dependent on global intraterminal [Ca2+] ([Ca2+]I). Surprisingly, coordinated multivesicular release persisted at Cplx3−/− synapses, although its onset was slowed. Light-dependent signaling at Cplx3−/− RB→AII synapses was sluggish, owing largely to increased asynchronous release at light offset. Consequently, propagation of RB output to retinal ganglion cells was suppressed dramatically. Our study links Cplx3 expression with synapse and circuit function in a specific retinal pathway and reveals a role for asynchronous release in circuit gain control.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 15, Issue 10, 7 June 2016, Pages 2239–2250
نویسندگان
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