کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2039322 1073047 2015 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Low-Cell-Number Epigenome Profiling Aids the Study of Lens Aging and Hematopoiesis
ترجمه فارسی عنوان
اسیدهای چرب اشباع شده با کمترین تعداد سلولهای عصبی، مطالعه پیری لنز و هماتوپوئزی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی


• RP-ChIP-seq enables high-fidelity epigenetic profiling in 500 cells
• FARP-ChIP-seq is generally applicable
• Age-associated epigenome changes in mouse lens are revealed by RP-ChIP-seq
• Lack of H3K4me3/H3K27me3 bivalency on hematopoietic differentiation genes in HSCs

SummaryUnderstanding how chromatin modification regulates development and disease can be limited by available material. Despite recent progress, balancing high-quality and reliable mapping using chromatin-immunoprecipitation-based deep sequencing (ChIP-seq) remains a challenge. We report two techniques, recovery via protection (RP)-ChIP-seq and favored amplification RP-ChIP-seq (FARP-ChIP-seq), that provide reproducible mapping in as few as 500 cells. RP-ChIP-seq allows detection of age-associated epigenetic changes in a single mouse lens, whereas FARP-ChIP-seq accurately maps histone H3 lysine 4 trimethylation (H3K4me3) and H3K27me3 in long-term hematopoietic stem cells (LT-HSCs), short-term HSCs (ST-HSCs), and multi-potent progenitors (MPPs) from one mouse. These datasets not only highlight genes that may be involved in lens aging but also indicate a lack of H3K4me3/H3K27me3 bivalency on hematopoietic genes in HSCs.

Graphical AbstractFigure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 13, Issue 7, 17 November 2015, Pages 1505–1518
نویسندگان
, , , , , ,