کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2039322 | 1073047 | 2015 | 14 صفحه PDF | دانلود رایگان |
• RP-ChIP-seq enables high-fidelity epigenetic profiling in 500 cells
• FARP-ChIP-seq is generally applicable
• Age-associated epigenome changes in mouse lens are revealed by RP-ChIP-seq
• Lack of H3K4me3/H3K27me3 bivalency on hematopoietic differentiation genes in HSCs
SummaryUnderstanding how chromatin modification regulates development and disease can be limited by available material. Despite recent progress, balancing high-quality and reliable mapping using chromatin-immunoprecipitation-based deep sequencing (ChIP-seq) remains a challenge. We report two techniques, recovery via protection (RP)-ChIP-seq and favored amplification RP-ChIP-seq (FARP-ChIP-seq), that provide reproducible mapping in as few as 500 cells. RP-ChIP-seq allows detection of age-associated epigenetic changes in a single mouse lens, whereas FARP-ChIP-seq accurately maps histone H3 lysine 4 trimethylation (H3K4me3) and H3K27me3 in long-term hematopoietic stem cells (LT-HSCs), short-term HSCs (ST-HSCs), and multi-potent progenitors (MPPs) from one mouse. These datasets not only highlight genes that may be involved in lens aging but also indicate a lack of H3K4me3/H3K27me3 bivalency on hematopoietic genes in HSCs.
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Journal: - Volume 13, Issue 7, 17 November 2015, Pages 1505–1518