کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2039349 1400967 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Unique Requirement for ESCRT Factors in Flavivirus Particle Formation on the Endoplasmic Reticulum
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Unique Requirement for ESCRT Factors in Flavivirus Particle Formation on the Endoplasmic Reticulum
چکیده انگلیسی


• Several ESCRT subunits are recruited to sites of flavivirus replication on the ER
• Systematic siRNA screening identifies a unique requirement for ESCRT factors
• Essential ESCRT subunits, VPS4 and ALIX, are dispensable for flavivirus replication
• ESCRT depletion affects virual budding but not the genome replication

SummaryFlavivirus infection induces endoplasmic reticulum (ER) membrane rearrangements to generate a compartment for replication of the viral genome and assembly of viral particles. Using quantitative mass spectrometry, we identified several ESCRT (endosomal sorting complex required for transport) proteins that are recruited to sites of virus replication on the ER. Systematic small interfering RNA (siRNA) screening revealed that release of both dengue virus and Japanese encephalitis virus was dramatically decreased by single depletion of TSG101 or co-depletion of specific combinations of ESCRT-III proteins, resulting in ≥1,000-fold titer reductions. By contrast, release was unaffected by depletion of some core ESCRTs, including VPS4. Reintroduction of ESCRT proteins to siRNA-depleted cells revealed interactions among ESCRT proteins that are crucial for flavivirus budding. Electron-microscopy studies revealed that the CHMP2 and CHMP4 proteins function directly in membrane deformation at the ER. Thus, a unique and specific subset of ESCRT contributes to ER membrane biogenesis during flavivirus infection.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 16, Issue 9, 30 August 2016, Pages 2339–2347
نویسندگان
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