Iron Toxicity in the Retina Requires Alu RNA and the NLRP3 Inflammasome

• Iron overload induces retinal pigmented epithelium death via NLRP3 inflammasome
• SINE RNAs are intermediates of iron-induced inflammasome activation and cell death
• Iron promotes SINE RNA accumulation by inhibiting DICER1 activity
• Efficient DICER1 processing of SINE RNAs by PCBP2 is inhibited by iron overload

SummaryExcess iron induces tissue damage and is implicated in age-related macular degeneration (AMD). Iron toxicity is widely attributed to hydroxyl radical formation through Fenton’s reaction. We report that excess iron, but not other Fenton catalytic metals, induces activation of the NLRP3 inflammasome, a pathway also implicated in AMD. Additionally, iron-induced degeneration of the retinal pigmented epithelium (RPE) is suppressed in mice lacking inflammasome components caspase-1/11 or Nlrp3 or by inhibition of caspase-1. Iron overload increases abundance of RNAs transcribed from short interspersed nuclear elements (SINEs): Alu RNAs and the rodent equivalent B1 and B2 RNAs, which are inflammasome agonists. Targeting Alu or B2 RNA prevents iron-induced inflammasome activation and RPE degeneration. Iron-induced SINE RNA accumulation is due to suppression of DICER1 via sequestration of the co-factor poly(C)-binding protein 2 (PCBP2). These findings reveal an unexpected mechanism of iron toxicity, with implications for AMD and neurodegenerative diseases associated with excess iron.

Graphical AbstractFigure optionsDownload as PowerPoint slide