کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2039611 1073070 2016 15 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Glycosaminoglycan Profiling in Patients’ Plasma and Urine Predicts the Occurrence of Metastatic Clear Cell Renal Cell Carcinoma
ترجمه فارسی عنوان
گلیکواسامینوگلیکان در بیماران؟ پلاسما و ادرار احتمال ابتلا به سرطان سلول های کلیه سلول های بنیادی متاستاتیک را پیش بینی می کند
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی


• Coordinated regulation of glycosaminoglycan (GAG) biosynthesis is observed in ccRCC
• The plasma and urine GAG profile is strongly altered in metastatic ccRCC
• Three non-invasive GAG scores accurately predict the occurrence of metastatic ccRCC
• The GAG scores normalize in patients with no evidence of disease

SummaryMetabolic reprogramming is a hallmark of clear cell renal cell carcinoma (ccRCC) progression. Here, we used genome-scale metabolic modeling to elucidate metabolic reprogramming in 481 ccRCC samples and discovered strongly coordinated regulation of glycosaminoglycan (GAG) biosynthesis at the transcript and protein levels. Extracellular GAGs are implicated in metastasis, so we speculated that such regulation might translate into a non-invasive biomarker for metastatic ccRCC (mccRCC). We measured 18 GAG properties in 34 mccRCC samples versus 16 healthy plasma and/or urine samples. The GAG profiles were distinctively altered in mccRCC. We derived three GAG scores that distinguished mccRCC patients with 93.1%–100% accuracy. We validated the score accuracies in an independent cohort (up to 18 mccRCC versus nine healthy) and verified that the scores normalized in eight patients with no evidence of disease. In conclusion, coordinated regulation of GAG biosynthesis occurs in ccRCC, and non-invasive GAG profiling is suitable for mccRCC diagnosis.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 15, Issue 8, 24 May 2016, Pages 1822–1836
نویسندگان
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