کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2039650 | 1073073 | 2014 | 12 صفحه PDF | دانلود رایگان |
• C. elegans MAFR-1 regulates both RNA Pol II- and III-dependent transcription
• MAFR-1 levels impact de novo lipogenesis gene expression and lipid levels
• Dietary carbohydrates and insulin signaling regulate mafr-1 levels
• MAFR-1 cell nonautonomously affects oogenesis and reproductive output
SummaryMaf1 is a conserved repressor of RNA polymerase (Pol) III transcription; however, its physiological role in the context of a multicellular organism is not well understood. Here, we show that C. elegans MAFR-1 is functionally orthologous to human Maf1, represses the expression of both RNA Pol III and Pol II transcripts, and mediates organismal fecundity and lipid homeostasis. MAFR-1 impacts lipid transport by modulating intestinal expression of the vitellogenin family of proteins, resulting in cell-nonautonomous defects in the developing reproductive system. MAFR-1 levels inversely correlate with stored intestinal lipids, in part by influencing the expression of the lipogenesis enzymes fasn-1/FASN and pod-2/ACC1. Animals fed a high carbohydrate diet exhibit reduced mafr-1 expression and mutations in the insulin signaling pathway genes daf-18/PTEN and daf-16/FoxO abrogate the lipid storage defects associated with deregulated mafr-1 expression. Our results reveal physiological roles for mafr-1 in regulating organismal lipid homeostasis, which ensure reproductive success.
Graphical AbstractFigure optionsDownload as PowerPoint slide
Journal: - Volume 9, Issue 6, 24 December 2014, Pages 2180–2191