کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2039851 1073086 2015 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Organization of Mitochondrial Gene Expression in Two Distinct Ribosome-Containing Assemblies
ترجمه فارسی عنوان
سازماندهی بیان ژن میتوکندری در دو مجموعه مجزا از ریبوزوم
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی


• Mitochondrial ribosomes have a large interactome, resulting in MIOREX complexes
• MIOREX complexes organize ribosomes and mRNA metabolism in large assemblies
• A subset of the MIOREX complexes is associated with the nucleoid
• MIOREX complexes channel gene expression from transcription to translation

SummaryMitochondria contain their own genetic system that provides subunits of the complexes driving oxidative phosphorylation. A quarter of the mitochondrial proteome participates in gene expression, but how all these factors are orchestrated and spatially organized is currently unknown. Here, we established a method to purify and analyze native and intact complexes of mitochondrial ribosomes. Quantitative mass spectrometry revealed extensive interactions of ribosomes with factors involved in all the steps of posttranscriptional gene expression. These interactions result in large expressosome-like assemblies that we termed mitochondrial organization of gene expression (MIOREX) complexes. Superresolution microscopy revealed that most MIOREX complexes are evenly distributed throughout the mitochondrial network, whereas a subset is present as nucleoid-MIOREX complexes that unite the whole spectrum of organellar gene expression. Our work therefore provides a conceptual framework for the spatial organization of mitochondrial protein synthesis that likely developed to facilitate gene expression in the organelle.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 10, Issue 6, 17 February 2015, Pages 843–853
نویسندگان
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