کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2040131 | 1073099 | 2016 | 7 صفحه PDF | دانلود رایگان |
• An augmin knockout (KO) mouse is used to examine microtubule nucleation
• Augmin is essential for embryonic cell division during mouse development
• Live imaging of synchronized embryos reveals augmin-dependent clustering of MTOCs
• Intra-spindle MT assembly, but not augmin itself, is required for MTOC clustering
SummaryErrors during cell division in oocytes and early embryos are linked to birth defects in mammals. Bipolar spindle assembly in early mouse embryos is unique in that three or more acentriolar microtubule-organizing centers (MTOCs) are initially formed and are then clustered into two spindle poles. Using a knockout mouse and live imaging of spindles in embryos, we demonstrate that MTOC clustering during the blastocyst stage requires augmin, a critical complex for MT-dependent MT nucleation within the spindle. Functional analyses in cultured cells with artificially increased numbers of centrosomes indicate that the lack of intra-spindle MT nucleation, but not loss of augmin per se or overall reduction of spindle MTs, is the cause of clustering failure. These data suggest that onset of mitosis with three or more MTOCs is turned into a typical bipolar division through augmin-dependent intra-spindle MT assembly.
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Journal: - Volume 15, Issue 1, 5 April 2016, Pages 54–60