کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2040137 1073099 2016 15 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
PRRT2 Is a Key Component of the Ca2+-Dependent Neurotransmitter Release Machinery
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
PRRT2 Is a Key Component of the Ca2+-Dependent Neurotransmitter Release Machinery
چکیده انگلیسی


• PRRT2 is a presynaptic protein
• PRRT2 is required for synchronous neurotransmitter release
• PRRT2 silencing decreases synaptic density and release probability
• PRRT2 interacts with the fast Ca2+ sensors synaptotagmin 1/2

SummaryHeterozygous mutations in proline-rich transmembrane protein 2 (PRRT2) underlie a group of paroxysmal disorders, including epilepsy, kinesigenic dyskinesia, and migraine. Most of the mutations lead to impaired PRRT2 expression, suggesting that loss of PRRT2 function may contribute to pathogenesis. We show that PRRT2 is enriched in presynaptic terminals and that its silencing decreases the number of synapses and increases the number of docked synaptic vesicles at rest. PRRT2-silenced neurons exhibit a severe impairment of synchronous release, attributable to a sharp decrease in release probability and Ca2+ sensitivity and associated with a marked increase of the asynchronous/synchronous release ratio. PRRT2 interacts with the synaptic proteins SNAP-25 and synaptotagmin 1/2. The results indicate that PRRT2 is intimately connected with the Ca2+-sensing machinery and that it plays an important role in the final steps of neurotransmitter release.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 15, Issue 1, 5 April 2016, Pages 117–131
نویسندگان
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