BRCA1 Accelerates CtIP-Mediated DNA-End Resection

• A high-resolution method is used to measure DNA-end resection
• BRCA1 interaction with CtIP is not essential for resection
• BRCA1 interaction with CtIP affects resection speed

SummaryDNA-end resection is a highly regulated and critical step in the response and repair of DNA double-strand breaks. In higher eukaryotes, CtIP regulates resection by integrating cellular signals via its posttranslational modifications and protein-protein interactions, including cell-cycle-controlled interaction with BRCA1. The role of BRCA1 in DNA-end resection is not clear. Here, we develop an assay to study DNA resection in higher eukaryotes at high resolution. We demonstrate that the BRCA1-CtIP interaction, albeit not essential for resection, modulates the speed at which this process takes place.

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