Microprocessor Activity Controls Differential miRNA Biogenesis In Vivo

• In vivo insight into miRNA biogenesis using next-generation RNA sequencing
• Transcriptome-wide estimation of in vivo pri-miRNA processing efficiencies
• Differential Microprocessor cleavage as a key regulatory step in miRNA biogenesis
• Uncoupling of miRNA biogenesis from host gene expression by Microprocessor

SummaryIn miRNA biogenesis, pri-miRNA transcripts are converted into pre-miRNA hairpins. The in vivo properties of this process remain enigmatic. Here, we determine in vivo transcriptome-wide pri-miRNA processing using next-generation sequencing of chromatin-associated pri-miRNAs. We identify a distinctive Microprocessor signature in the transcriptome profile from which efficiency of the endogenous processing event can be accurately quantified. This analysis reveals differential susceptibility to Microprocessor cleavage as a key regulatory step in miRNA biogenesis. Processing is highly variable among pri-miRNAs and a better predictor of miRNA abundance than primary transcription itself. Processing is also largely stable across three cell lines, suggesting a major contribution of sequence determinants. On the basis of differential processing efficiencies, we define functionality for short sequence features adjacent to the pre-miRNA hairpin. In conclusion, we identify Microprocessor as the main hub for diversified miRNA output and suggest a role for uncoupling miRNA biogenesis from host gene expression.

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