کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2040245 1073103 2014 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cellular Heterogeneity in the Mouse Esophagus Implicates the Presence of a Nonquiescent Epithelial Stem Cell Population
ترجمه فارسی عنوان
ناهمگن سلولی در مغز موش صحت وجود جمعیت سلول بنیادی اپیتلیال
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی


• 3D esophageal organoids generated in vitro reflect in vivo tissue architecture
• Cell-surface markers separate subpopulations of mouse esophageal basal epithelium
• Subpopulations have distinct differentiation and proliferation properties
• A nonquiescent stem cell population resides in the basal layer of the esophagus

SummaryBecause the esophageal epithelium lacks a defined stem cell niche, it is unclear whether all basal epithelial cells in the adult esophagus are functionally equivalent. In this study, we showed that basal cells in the mouse esophagus contained a heterogeneous population of epithelial cells, similar to other rapidly cycling tissues such as the intestine or skin. Using a combination of cell-surface markers, we separated primary esophageal tissue into distinct cell populations that harbored differences in stem cell potential. We also used an in vitro 3D organoid assay to demonstrate that Sox2, Wnt, and bone morphogenetic protein signaling regulate esophageal self-renewal. Finally, we labeled proliferating basal epithelial cells in vivo to show differing cell-cycle profiles and proliferation kinetics. Based on our results, we propose that a nonquiescent stem cell population resides in the basal epithelium of the mouse esophagus.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 9, Issue 2, 23 October 2014, Pages 701–711
نویسندگان
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