کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2040333 1073107 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular Architecture of Transcription Factor Hotspots in Early Adipogenesis
ترجمه فارسی عنوان
معماری مولکولی نقاط فاکتور رونویسی در آودپوژنز زودرس
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی


• Transcription factor (TF) footprints precisely localize proteins within hotspots
• A composite motif directs the binding of ATF-C/EBP heterodimers on chromatin
• TFs are frequently recruited to hotspots by mechanisms independent of their known motif
• Alternative mechanisms of C/EBPβ recruitment to hotspots are functionally important

SummaryTranscription factors have recently been shown to colocalize in hotspot regions of the genome, which are further clustered into super-enhancers. However, the detailed molecular organization of transcription factors at hotspot regions is poorly defined. Here, we have used digital genomic footprinting to precisely define factor localization at a genome-wide level during the early phase of 3T3-L1 adipocyte differentiation, which allows us to obtain detailed molecular insight into how transcription factors target hotspots. We demonstrate the formation of ATF-C/EBP heterodimers at a composite motif on chromatin, and we suggest that this may be a general mechanism for integrating external signals on chromatin. Furthermore, we find evidence of extensive recruitment of transcription factors to hotspots through alternative mechanisms not involving their known motifs and demonstrate that these alternative binding events are functionally important for hotspot formation and activity. Taken together, these findings provide a framework for understanding transcription factor cooperativity in hotspots.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 7, Issue 5, 12 June 2014, Pages 1434–1442
نویسندگان
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