کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2040450 | 1073111 | 2015 | 13 صفحه PDF | دانلود رایگان |
• Sensitive RNA-seq approach detects tissue-specific maternal and paternal allele biases
• Noncanonical imprinting effects are conserved in wild populations
• Nascent RNA in situ hybridization reveals allelic effects in subpopulations of neurons
• Noncanonical imprinting influences the monoamine pathway and offspring behavior
SummaryHere, we describe an RNA-sequencing (RNA-seq)-based approach that accurately detects even modest maternal or paternal allele expression biases at the tissue level, which we call noncanonical genomic imprinting effects. We profile imprinting in the arcuate nucleus (ARN) and dorsal raphe nucleus of the female mouse brain as well as skeletal muscle (mesodermal) and liver (endodermal). Our study uncovers hundreds of noncanonical autosomal and X-linked imprinting effects. Noncanonical imprinting is highly tissue-specific and enriched in the ARN, but rare in the liver. These effects are reproducible across different genetic backgrounds and associated with allele-specific chromatin. Using in situ hybridization for nascent RNAs, we discover that autosomal noncanonical imprinted genes with a tissue-level allele bias exhibit allele-specific expression effects in subpopulations of neurons in the brain in vivo. We define noncanonical imprinted genes that regulate monoamine signaling and determine that these effects influence the impact of inherited mutations on offspring behavior.
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Journal: - Volume 12, Issue 6, 11 August 2015, Pages 979–991