Septin-Containing Barriers Control the Differential Inheritance of Cytoplasmic Elements

SummaryFusion of haploid cells of Saccharomyces cerevisiae generates zygotes. We observe that the zygote midzone includes a septin annulus and differentially affects redistribution of supramolecular complexes and organelles. Redistribution across the midzone of supramolecular complexes (polysomes and Sup35p-GFP [PSI+]) is unexpectedly delayed relative to soluble proteins; however, in [psi-] × [PSI+] crosses, all buds eventually receive Sup35p-GFP [PSI+]. Encounter between parental mitochondria is further delayed until septins relocate to the bud site, where they are required for repolarization of the actin cytoskeleton. This delay allows rationalization of the longstanding observation that terminal zygotic buds preferentially inherit a single mitochondrial genotype. The rate of redistribution of complexes and organelles determines whether their inheritance will be uniform.

Graphical AbstractFigure optionsDownload as PowerPoint slideHighlights
► Septin-containing structures partition the cytoplasm and reorganize actin
► These structures promote uniparental inheritance of yeast mitochondria
► Inhomogeneity of the cytoplasm accounts for asymmetric transgenerational inheritance