کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2041031 1073141 2012 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Inhibitory Effects of Robo2 on Nephrin: A Crosstalk between Positive and Negative Signals Regulating Podocyte Structure
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Inhibitory Effects of Robo2 on Nephrin: A Crosstalk between Positive and Negative Signals Regulating Podocyte Structure
چکیده انگلیسی

SummaryRobo2 is the cell surface receptor for the repulsive guidance cue Slit and is involved in axon guidance and neuronal migration. Nephrin is a podocyte slit-diaphragm protein that functions in the kidney glomerular filtration barrier. Here, we report that Robo2 is expressed at the basal surface of mouse podocytes and colocalizes with nephrin. Biochemical studies indicate that Robo2 forms a complex with nephrin in the kidney through adaptor protein Nck. In contrast to the role of nephrin that promotes actin polymerization, Slit2-Robo2 signaling inhibits nephrin-induced actin polymerization. In addition, the amount of F-actin associated with nephrin is increased in Robo2 knockout mice that develop an altered podocyte foot process structure. Genetic interaction study further reveals that loss of Robo2 alleviates the abnormal podocyte structural phenotype in nephrin null mice. These results suggest that Robo2 signaling acts as a negative regulator on nephrin to influence podocyte foot process architecture.

Graphical AbstractFigure optionsDownload as PowerPoint slideHighlights
► Robo2 colocalizes with nephrin in kidney podocytes
► Robo2 forms a complex with nephrin through the Nck protein
► Slit2-Robo2 signaling inhibits nephrin-induced actin polymerization
► Robo2 loss alleviates podocyte structural phenotype in nephrin mutant mice

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 2, Issue 1, 26 July 2012, Pages 52–61
نویسندگان
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