کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2041214 | 1073151 | 2016 | 15 صفحه PDF | دانلود رایگان |
• Absolute quantification of the cell proteome during human erythroid differentiation
• Comparison of transcriptome and proteome modifications during erythropoiesis
• Distribution of 1,300 proteins between reticulocytes and pyrenocytes after enucleation
SummaryMass spectrometry-based proteomics now enables the absolute quantification of thousands of proteins in individual cell types. We used this technology to analyze the dynamic proteome changes occurring during human erythropoiesis. We quantified the absolute expression of 6,130 proteins during erythroid differentiation from late burst-forming units-erythroid (BFU-Es) to orthochromatic erythroblasts. A modest correlation between mRNA and protein expression was observed. We identified several proteins with unexpected expression patterns in erythroid cells, highlighting a breakpoint in the erythroid differentiation process at the basophilic stage. We also quantified the distribution of proteins between reticulocytes and pyrenocytes after enucleation. These analyses identified proteins that are actively sorted either with the reticulocyte or the pyrenocyte. Our study provides the absolute quantification of protein expression during a complex cellular differentiation process in humans, and it establishes a framework for future studies of disordered erythropoiesis.
Graphical AbstractFigure optionsDownload as PowerPoint slide
Journal: - Volume 16, Issue 5, 2 August 2016, Pages 1470–1484