کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2041328 1073156 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Modularized Functions of the Fanconi Anemia Core Complex
ترجمه فارسی عنوان
توابع مدولار مجتمع هسته آنمی فانکونی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی


• FA gene products that form the E3 ligase catalytic core module are identified
• FANCG/FANCF-containing modules mediate DNA damage recruitment of the catalytic module
• A model of modularized functional assembly of the FA core complex is proposed

SummaryThe Fanconi anemia (FA) core complex provides the essential E3 ligase function for spatially defined FANCD2 ubiquitination and FA pathway activation. Of the seven FA gene products forming the core complex, FANCL possesses a RING domain with demonstrated E3 ligase activity. The other six components do not have clearly defined roles. Through epistasis analyses, we identify three functional modules in the FA core complex: a catalytic module consisting of FANCL, FANCB, and FAAP100 is absolutely required for the E3 ligase function, and the FANCA-FANCG-FAAP20 and the FANCC-FANCE-FANCF modules provide nonredundant and ancillary functions that help the catalytic module bind chromatin or sites of DNA damage. Disruption of the catalytic module causes complete loss of the core complex function, whereas loss of any ancillary module component does not. Our work reveals the roles of several FA gene products with previously undefined functions and a modularized assembly of the FA core complex.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 7, Issue 6, 26 June 2014, Pages 1849–1857
نویسندگان
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