کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2041379 1073158 2015 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Mechanism and Regulation of Rapid Telomere Prophase Movements in Mouse Meiotic Chromosomes
ترجمه فارسی عنوان
مکانیزم و تنظیم حرکات سریع پروتئین تلومر در کروموزوم های میوتیک ماوس
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی


• Direct visualization of chromosome movements is seen in live mouse seminiferous tubules
• Quantitative analysis revealed characteristics and regulation of RPMs
• SUN1/KASH5 connects telomere to dynein on cytoskeletal microtubules to generate RPMs

SummaryTelomere-led rapid prophase movements (RPMs) in meiotic prophase have been observed in diverse eukaryote species. A shared feature of RPMs is that the force that drives the chromosomal movements is transmitted from the cytoskeleton, through the nuclear envelope, to the telomeres. Studies in mice suggested that dynein movement along microtubules is transmitted to telomeres through SUN1/KASH5 nuclear envelope bridges to generate RPMs. We monitored RPMs in mouse seminiferous tubules using 4D fluorescence imaging and quantitative motion analysis to characterize patterns of movement in the RPM process. We find that RPMs reflect a combination of nuclear rotation and individual chromosome movements. The telomeres move along microtubule tracks that are apparently continuous with the cytoskeletal network and exhibit characteristic arrangements at different stages of prophase. Quantitative measurements confirmed that SUN1/KASH5, microtubules, and dynein, but not actin, were necessary for RPMs and that defects in meiotic recombination and synapsis resulted in altered RPMs.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 11, Issue 4, 28 April 2015, Pages 551–563
نویسندگان
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