کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2041540 | 1073165 | 2013 | 11 صفحه PDF | دانلود رایگان |
• Teneurin-3 is expressed by RGCs, amacrine cells, and tectal neurons
• Teneurin-3 is required by RGC dendrites for correct IPL stratification
• A subset of RGC axons show laminar arborization defects upon Teneurin-3 knockdown
• Orientation-, but not direction-selective, RGCs are impaired in Teneurin-3 morphants
SummaryA striking feature of the CNS is the precise wiring of its neuronal connections. During vertebrate visual system development, different subtypes of retinal ganglion cells (RGCs) form specific connections with their corresponding synaptic partners. However, the underlying molecular mechanisms remain to be fully elucidated. Here, we report that the cell-adhesive transmembrane protein Teneurin-3 (Tenm3) is required by zebrafish RGCs for acquisition of their correct morphological and functional connectivity in vivo. Teneurin-3 is expressed by RGCs and their presynaptic amacrine and postsynaptic tectal cell targets. Knockdown of Teneurin-3 leads to RGC dendrite stratification defects within the inner plexiform layer, as well as mistargeting of dendritic processes into outer portions of the retina. Moreover, a subset of RGC axons exhibits tectal laminar arborization errors. Finally, functional analysis of RGCs targeting the tectum reveals a selective deficit in the development of orientation selectivity after Teneurin-3 knockdown. These results suggest that Teneurin-3 plays an instructive role in the functional wiring of the vertebrate visual system.
Graphical AbstractFigure optionsDownload as PowerPoint slide
Journal: - Volume 5, Issue 3, 14 November 2013, Pages 582–592