کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2041545 | 1073165 | 2013 | 8 صفحه PDF | دانلود رایگان |
• Two models for X chromosome dosage compensation in Drosophila are tested
• Pol II recruitment and pausing are analyzed by GRO-seq and small 5′ RNA sequencing
• Elongation is measured by direct RNA sequencing of nascent transcripts
• Results support a transcriptional elongation model for dosage compensation
SummaryDosage compensation in Drosophila is mediated by the MSL complex, which increases male X-linked gene expression approximately 2-fold. The MSL complex preferentially binds the bodies of active genes on the male X, depositing H4K16ac with a 3′ bias. Two models have been proposed for the influence of the MSL complex on transcription: one based on promoter recruitment of RNA polymerase II (Pol II), and a second featuring enhanced transcriptional elongation. Here, we utilize nascent RNA sequencing to document dosage compensation during transcriptional elongation. We also compare X and autosomes from published data on paused and elongating polymerase in order to assess the role of Pol II recruitment. Our results support a model for differentially regulated elongation, starting with release from 5′ pausing and increasing through X-linked gene bodies. Our results highlight facilitated transcriptional elongation as a key mechanism for the coordinated regulation of a diverse set of genes.
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Journal: - Volume 5, Issue 3, 14 November 2013, Pages 629–636