کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2041666 1073169 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Circulation-Independent Differentiation Pathway from Extraembryonic Mesoderm toward Hematopoietic Stem Cells via Hemogenic Angioblasts
ترجمه فارسی عنوان
مسیرهای تمایز گردش خون مستقل از مزودرم اکسترومبرونیک به سلول های بنیادی هماتوپوئیدی از طریق آنژیوابلاست های هموژن
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی


• Two Runx1+ populations subdivided by Gata1 expression exist in E7.5 embryos
• E7.5 Runx1+Gata1− cells (R+G− cells) contribute to HSC lineage and endothelium
• E7.5 R+G− cells migrate from extra- to intraembryonic site at precirculation stage
• E7.5 R+G− cells that contribute to intraembryonic endothelium have hemogenic potential

SummaryA large gap exists in our understanding of the course of differentiation from mesoderm to definitive hematopoietic stem cells (HSCs). Previously, we reported that Runx1+ cells in embryonic day 7.5 (E7.5) embryos contribute to the hemogenic endothelium in the E10.5 aorta-gonad-mesonephros (AGM) region and HSCs in the adult bone marrow. Here, we show that two Runx1+ populations subdivided by Gata1 expression exist in E7.5 embryos. The hemogenic endothelium and the HSCs are derived only from the Runx1+Gata1− population. A subset of this population moves from the extra- to intraembryonic region during E7.5–E8.0, where it contributes to the hemogenic endothelium of the dorsal aorta (DA). Migration occurs before the heartbeat is initiated, and it is independent of circulation. This suggests a developmental trajectory from Runx1+ cells in the E7.5 extraembryonic region to definitive HSCs via the hemogenic endothelium.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 8, Issue 1, 10 July 2014, Pages 31–39
نویسندگان
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