Functional Genomic Analysis of Human Mitochondrial RNA Processing

• MitoString directly measures mtDNA-encoded precursor and mature RNA species
• MitoString profiles are obtained after silencing of 107 mtRNA-binding factors
• MitoString provides insights into the roles of MRPP1–MRPP3, ELAC2, LRPPRC, and SLIRP
• FASTKD4 associates with mt-mRNAs and stabilizes a subset of them

SummaryBoth strands of human mtDNA are transcribed in continuous, multigenic units that are cleaved into the mature rRNAs, tRNAs, and mRNAs required for respiratory chain biogenesis. We sought to systematically identify nuclear-encoded proteins that contribute to processing of mtRNAs within the organelle. First, we devised and validated a multiplex MitoString assay that quantitates 27 mature and precursor mtDNA transcripts. Second, we applied MitoString profiling to evaluate the impact of silencing each of 107 mitochondrial-localized, predicted RNA-binding proteins. With the resulting data set, we rediscovered the roles of recently identified RNA-processing enzymes, detected unanticipated roles of known disease genes in RNA processing, and identified new regulatory factors. We demonstrate that one such factor, FASTKD4, modulates the half-lives of a subset of mt-mRNAs and associates with mtRNAs in vivo. MitoString profiling may be useful for diagnosing and deciphering the pathogenesis of mtDNA disorders.

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