کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2042074 1073185 2015 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Nucleosome Assembly Dynamics Involve Spontaneous Fluctuations in the Handedness of Tetrasomes
ترجمه فارسی عنوان
دینامیک مجتمع هسته ای شامل نوسانات خودانگیختگی در تتراسوم ها می شود
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی


• We monitor assembly of nucleosomes and tetrasomes by NAP1 on DNA in real time
• Tetrasomes spontaneously flip between a left- and right-handed conformation
• Addition of H2A/H2B to flipping tetrasomes generates stable left-handed nucleosomes
• Small positive torques drive tetrasomes from a left-handed into a right-handed state

SummaryDNA wrapping around histone octamers generates nucleosomes, the basic compaction unit of eukaryotic chromatin. Nucleosome stability is carefully tuned to maintain DNA accessibility in transcription, replication, and repair. Using freely orbiting magnetic tweezers, which measure the twist and length of single DNA molecules, we monitor the real-time loading of tetramers or complete histone octamers onto DNA by Nucleosome Assembly Protein-1 (NAP1). Remarkably, we find that tetrasomes exhibit spontaneous flipping between a preferentially occupied left-handed state (ΔLk = −0.73) and a right-handed state (ΔLk = +1.0), separated by a free energy difference of 2.3 kBT (1.5 kcal/mol). This flipping occurs without concomitant changes in DNA end-to-end length. The application of weak positive torque converts left-handed tetrasomes into right-handed tetrasomes, whereas nucleosomes display more gradual conformational changes. Our findings reveal unexpected dynamical rearrangements of the nucleosomal structure, suggesting that chromatin can serve as a “twist reservoir,” offering a mechanistic explanation for the regulation of DNA supercoiling in chromatin.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 10, Issue 2, 13 January 2015, Pages 216–225
نویسندگان
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