کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2042130 1073187 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Coupling Transcriptional State to Large-Scale Repeat Expansions in Yeast
ترجمه فارسی عنوان
دولت رونویسی اتصال به گسترش بزرگ تکرار در مخمر
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی


• Large-scale repeat expansions can occur in the lack of transcription
• Transcriptional activation of a locus elevates the rate of repeat expansions
• Replication defects affect expansions irrespective of a repeat's transcriptional state
• Nucleosome density acts as an inherent modulator of repeat expansions

SummaryExpansions of simple DNA repeats cause numerous hereditary disorders in humans. Replication, repair, and transcription are implicated in the expansion process, but their relative contributions are yet to be distinguished. To separate the roles of replication and transcription in the expansion of Friedreich’s ataxia (GAA)n repeats, we designed two yeast genetic systems that utilize a galactose-inducible GAL1 promoter but contain these repeats in either the transcribed or nontranscribed region of a selectable cassette. We found that large-scale repeat expansions can occur in the lack of transcription. Induction of transcription strongly elevated the rate of expansions in both systems, indicating that active transcriptional state rather than transcription through the repeat per se affects this process. Furthermore, replication defects increased the rate of repeat expansions irrespective of transcriptional state. We present a model in which transcriptional state, linked to the nucleosomal density of a region, acts as a modulator of large-scale repeat expansions.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 9, Issue 5, 11 December 2014, Pages 1594–1602
نویسندگان
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