کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2042201 1073189 2014 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A Simple Method for 3D Analysis of Immunolabeled Axonal Tracts in a Transparent Nervous System
ترجمه فارسی عنوان
یک روش ساده برای تجزیه و تحلیل سهبعدی سیستم های عصبی شفاف عصبی ایمنی با برچسب ایمنی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی


• Immunostaining and 3DISCO clearing: a powerful method for studying brain connections
• 3D analysis of axon guidance defects in midline mutant mice
• Unexpected roles for Slits and Netrin-1 in fasciculus retroflexus development

SummaryClearing techniques have been developed to transparentize mouse brains, thereby preserving 3D structure, but their complexity has limited their use. Here, we show that immunolabeling of axonal tracts followed by optical clearing with solvents (3DISCO) and light-sheet microscopy reveals brain connectivity in mouse embryos and postnatal brains. We show that the Robo3 receptor is selectively expressed by medial habenula axons forming the fasciculus retroflexus (FR) and analyzed the development of this commissural tract in mutants of the Slit/Robo and DCC/Netrin pathways. Netrin-1 and DCC are required to attract FR axons to the midline, but the two mutants exhibit specific and heterogeneous axon guidance defects. Moreover, floor-plate-specific deletion of Slit ligands with a conditional Slit2 allele perturbs not only midline crossing by FR axons but also their anteroposterior distribution. In conclusion, this method represents a unique and powerful imaging tool to study axonal connectivity in mutant mice.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 9, Issue 4, 20 November 2014, Pages 1191–1201
نویسندگان
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