Rapid Evolution of Culture-Impaired Bacteria during Adaptation to Biofilm Growth

• Biofilms strongly promote the evolution of culture-impaired bacteria
• Culture-impaired growth is due to LPS mutations that produce a lethal fitness trade-off
• In biofilms, the LPS mutations markedly increase bacterial fitness
• Outside biofilms, the LPS mutations sensitize cells to a self-produced antibiotic

SummaryBiofilm growth increases the fitness of bacteria in harsh conditions. However, bacteria from clinical and environmental biofilms can exhibit impaired growth in culture, even when the species involved are readily culturable and permissive conditions are used. Here, we show that culture-impaired variants of Pseudomonas aeruginosa arise rapidly and become abundant in laboratory biofilms. The culture-impaired phenotype is caused by mutations that alter the outer-membrane lipopolysaccharide structure. Within biofilms, the lipopolysaccharide mutations markedly increase bacterial fitness. However, outside the protected biofilm environment, the mutations sensitize the variants to killing by a self-produced antimicrobial agent. Thus, a biofilm-mediated adaptation produces a stark fitness trade-off that compromises bacterial survival in culture. Trade-offs like this could limit the ability of bacteria to transition between biofilm growth and the free-living state and produce bacterial populations that escape detection by culture-based sampling.

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