کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
2055128 1075729 2016 6 صفحه PDF سفارش دهید دانلود رایگان
عنوان انگلیسی مقاله ISI
Limited artemisinin resistance-associated polymorphisms in Plasmodium falciparum K13-propeller and PfATPase6 gene isolated from Bioko Island, Equatorial Guinea
ترجمه فارسی عنوان
پلی مورفیسم مرتبط با مقاومت آرتمیسینین محدود در پروانه K13 پلاسمودیوم فالسیپاروم و ژن PfATPase6 جدا شده از جزیره بیکو، گینه استوایی
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کلمات کلیدی
پلاسمودیوم فالسیپاروم؛ پلی مورفیسم؛ مقاومت آرتمیسینین؛ پروانه K13
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی


• The PfATPase6 mutations were found at positions N569K (7.91%), A630S (1.44%) and I723V (0.72%).
• Only one non-synonymous K13-propeller mutation A578S (2.04%) was observed.
• Limited SNPs of PfATPase6 and K13-propeller indicated no widespread ART on Bioko Island, EG.

ObjectiveWith emergence and geographically expanding of antimalarial resistance worldwide, molecular markers are essential tool for surveillance of resistant Plasmodium parasites. Recently, single-nucleotide polymorphisms (SNPs) in the PF3D7_1343700 kelch propeller (K13-propeller) domain are shown to be associated with artemisinin (ART) resistance in vivo and in vitro. This study aims to investigate the ART resistance-associated polymorphisms of K13-propeller and PfATPase6 genes in Plasmodium falciparum isolates from Bioko Island, Equatorial Guinea (EG).MethodsA total of 172 samples were collected from falciparum malaria patients on Bioko Island between 2013 and 2014. The polymorphisms of K13-propeller and PfATPase6 genes were analyzed by Nest-PCR and sequencing.ResultsSequences of K13-propeller and PfATPase6 were obtained from 90.74% (98/108) and 91.45% (139/152) samples, respectively. The 2.04% (2/98) cases had non-synonymous K13-propeller A578S mutation but no found the mutations associated with ART resistance in Southeast Asia. For PfATPase6, the mutations were found at positions N569K and A630S with the mutation prevalence of 7.91% (11/139) and 1.44% (2/139), respectively. In addition, a sample with the mixed type at position I723V was discovered (0.72%, 1/139).ConclusionsThis study initially offers an insight of K13-propeller and PfATPase6 polymorphisms on Bioko Island, EG. It suggests no widespread ART resistance or tolerance in the region, and might be helpful for developing and updating guidance for the use of ART-based combination therapies (ACTs).

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal for Parasitology: Drugs and Drug Resistance - Volume 6, Issue 1, April 2016, Pages 54–59
نویسندگان
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