کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2058784 1543968 2015 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of collagen content within picrosirius red stained paraffin-embedded tissue sections using fluorescence microscopy
ترجمه فارسی عنوان
تعیین محتوای کلاژن در بخشهای بافت پارافین رنگ آمیزی قرمز رنگی با استفاده از میکروسکوپ فلورسانس
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
چکیده انگلیسی

Picrosirius red (PSR) staining is a commonly used histological technique to visualize collagen in paraffin-embedded tissue sections. PSR stained collagen appears red in light microscopy. However it is largely unknown that PSR stained collagen also shows a red fluorescence, whereas live cells have a distinct green autofluorescence. Both emission patterns can be detected using standard filter sets as found in conventional fluorescence microscopes. Here we used digital image addition and subtraction to determine the relative area of the pure collagen and live cell content in heart tissue in a semi-automated process using standard software. This procedure, which considers empty spaces (holes) within the section, can be easily adapted to quantify the collagen and live cell areas in healthy or fibrotic tissues as aorta, lung, kidney or liver by semi-automated planimetry exemplified herein for infarcted heart tissue obtained from the mouse myocardial infarction model.
• Use of conventional PSR stained paraffin-embedded tissue sections for fluorescence analysis.
• PSR and autofluorescence images are used to calculate area of collagen and area of live cells in the tissue; empty spaces (holes) in tissue are considered.
• High throughput analysis of collagen and live cell content in tissue for statistical purposes.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: MethodsX - Volume 2, 2015, Pages 124–134
نویسندگان
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