کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2062879 1076630 2009 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Purification and partial characterization of proteinase inhibitors of equine seminal plasma
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Purification and partial characterization of proteinase inhibitors of equine seminal plasma
چکیده انگلیسی

SUMMARYThe aims of the study were 1/to isolate and identify equine seminal plasma proteinase inhibitors, 2/to evaluate their inhibitory potential, and 3/to test a correlation between protein concentration in seminal plasma supernatant (obtained after precipitation with 36% ammonium sulfate) and stallion sexual maturity. Seminal plasma proteins obtained from six stallions were chromatographed in a Superose 12 (FPLC system) column followed by C18 HPLC reverse-phase. Inhibition of trypsin amidase activity was evaluated in the collected fractions. Active proteins with a molecular mass of 6.3-7.0 kDa were identified using mass spectrometry. The older stallions showed a reduction in total seminal plasma protein concentration, but had similar concentrations of proteinase inhibitors (0.28+0.10 mg/ml) in seminal plasma supernatant. Different proteinase inhibitor isoforms were found in semen of all stallions which suggests that the isoforms may be used as biomarkers of individual animals.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Reproductive Biology - Volume 9, Issue 2, July 2009, Pages 151–160
نویسندگان
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