کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2063967 | 1544116 | 2016 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determination and characterization of destruxin production in Metarhizium anisopliae Tk6 and formulations for Aedes aegypti mosquitoes control at the field level
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
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چکیده انگلیسی
Destruxins, cyclic hexadepsipeptide toxins, secreted by the entomopathogenic fungus, Metarhizium anisopliae through extracellular synthesis. The present study reports a new approach for the analysis of DTXs produced by the fungal strain Metarhizium anisoliae Tk6, using FRIR-HPLC-LC-MS and H1 NMR. The results also showed that production of the major DTXs A, B, C, and E have to be determined in Czapek Dextrose (CD) liquid culture filtrate from 9 to 12 days post-inoculation. Purified DTX were further tested in bioassays to assess their effects of Aedes aegypti mosquitoes. The four major purified DTX compounds were found to cause a toxic effect on the larval developmental stages of mosquitoes with high mortality rates. However, DTX E outperformed the other three DTXs by causing the highest mortality three days after inoculation. This result gives an alternative approach of using DTXs in mosquitoes control and used as a new method for other pest management.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicon - Volume 120, 15 September 2016, Pages 89-96
Journal: Toxicon - Volume 120, 15 September 2016, Pages 89-96
نویسندگان
Keppanan Ravindran, Komivi Senyo Akutse, Sivaperumal Sivaramakrishnan, Liande Wang,