کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2064155 1544119 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterisation of three alpha-helical antimicrobial peptides from the venom of Scorpio maurus palmatus
ترجمه فارسی عنوان
شناسایی سه پپتیدهای ضد میکروبی آلفا-اسپریلیک از زهر اسکورپی موروس پالمیتوس
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
چکیده انگلیسی


• Three antimicrobial peptides were derived from the venom of Scorpio maurus palmatus.
• Two of these peptides, Smp24 and Smp43, have promising membrane disruptive activities.
• A hinge-helical-hinge conformation promotes prokaryotic specificity in scorpion-derived antimicrobial peptides.

Scorpion venoms provide a rich source of anti-microbial peptides. Here we characterise three from the venom of Scorpion maurus palmatus. Smp13 is biologically inactive, despite sharing homology with other antimicrobial peptides, probably because it lacks a typically charged structure. Both Smp-24 and Smp-43 have broad spectrum antimicrobial activity, disrupting bacterial membranes. In addition, there is evidence that Smp24 may inhibit DNA synthesis in Bacillus subtilis. Smp24 haemolysed red blood cells but in contrast, Smp43 was non-haemolytic. The introduction of a flexible Gly-Val-Gly hinge into the middle of Smp24 did not alter the haemolytic activity of Smp24 (as might have been predicted from earlier studies with Pandinin2 (Pin2), although C-terminal truncation of Smp-24 reduced its haemolytic activity, in agreement with earlier Pin 2 studies. Smp24 and its derivatives, as well as Smp-43, were all cytotoxic (ATP release assay) toward mammalian HepG2 liver cells. Our results highlight the beneficial effect of helical-hinge-helical conformation on promoting prokaryotic selectivity of long chain scorpion AMPs, as well as the importance of examining a wide range of mammalian cell types in cytotoxicity testing.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicon - Volume 117, July 2016, Pages 30–36
نویسندگان
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