کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2064204 | 1544120 | 2016 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Bee venom phospholipase A2 as a membrane-binding vector for cell surface display or internalization of soluble proteins
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Bee venom phospholipase A2 as a membrane-binding vector for cell surface display or internalization of soluble proteins Bee venom phospholipase A2 as a membrane-binding vector for cell surface display or internalization of soluble proteins](/preview/png/2064204.png)
چکیده انگلیسی
We showed that bee venom phospholipase A2 can be used as a membrane-binding vector to anchor to the surface of cells a soluble protein fused to its C-terminus. ZZ, a two-domain derivative of staphylococcal protein A capable of binding constant regions of antibodies was fused to the C-terminus of the phospholipase or to a mutant devoid of enzymatic activity. The fusion proteins bound to the surface of cells and could themselves bind IgGs. Their fate depended on the cell type to which they bound. On the A431 carcinoma cell line the proteins remained exposed on the cell surface. In contrast, on human dendritic cells the proteins were internalized into early endosomes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicon - Volume 116, 15 June 2016, Pages 56–62
Journal: Toxicon - Volume 116, 15 June 2016, Pages 56–62
نویسندگان
Aurélie Babon, Thibault Wurceldorf, Christine Almunia, Sylvain Pichard, Alexandre Chenal, Cécile Buhot, Bruno Beaumelle, Daniel Gillet,