کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2064626 | 1544154 | 2014 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Detection of microvasculature alterations by synchrotron radiation in murine with delayed jellyfish envenomation syndrome Detection of microvasculature alterations by synchrotron radiation in murine with delayed jellyfish envenomation syndrome](/preview/png/2064626.png)
• We have previously established a delayed jellyfish envenomation syndrome (DJES) model.
• We used a third-generation synchrotron radiation facility.
• For the first time, we directly observed the blood vessel alterations induced by jellyfish venom.
Using the tentacle extract (TE) from the jellyfish Cyanea capillata, we have previously established a delayed jellyfish envenomation syndrome (DJES) model, which is meaningful for clinical interventions against jellyfish stings. However, the mechanism of DJES still remains unclear. Thus, this study aimed to explore its potential mechanism by detecting TE-induced microvasculature alterations in vivo and ex vivo. Using a third-generation synchrotron radiation facility, we, for the first time, directly observed the blood vessel alterations induced by jellyfish venom in vivo and ex vivo. Firstly, microvasculature imaging of whole-body mouse in vivo indicated that the small blood vessel branches in the liver and kidney in the TE-treated group, seemed much thinner than those in the control group. Secondly, 3D imaging of kidney ex vivo showed that the kidneys in the TE-treated group had incomplete vascular trees where distal vessel branches were partly missing and disorderly disturbed. Finally, histopathological analysis found that obvious morphological changes, especially hemorrhagic effects, were also present in the TE-treated kidney. Thus, TE-induced microvasculature changes might be one of the important mechanisms of multiple organ dysfunctions in DJES. In addition, the methods we employed here will probably facilitate further studies on developing effective intervention strategies against DJES.
Journal: Toxicon - Volume 81, April 2014, Pages 48–53