کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2064663 1544149 2014 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ciguatera fish poisoning in Hong Kong–A 10-year perspective on the class of ciguatoxins
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
Ciguatera fish poisoning in Hong Kong–A 10-year perspective on the class of ciguatoxins
چکیده انگلیسی


• We used LC-MS/MS to study retrospectively 10-year CTX-positive fish samples as determined by MBA.
• Pacific CTXs were the most commonly observed toxins found in the samples collected in Hong Kong.
• Pacific Ocean areas should be the important origin of CFP encountered.
• Clinical diagnosis from ciguatera patients supported intoxication of Pacific origin.
• Promising correlations were noted between toxicities derived by LC-MS/MS and MBA.

The present study used liquid chromatography-tandem mass spectrometry (LC-MS/MS) to investigate retrospectively ciguatoxin (CTX)-positive samples as determined by mouse bioassay (MBA) in the past 10 years in Hong Kong. The results showed that Pacific CTXs (P-CTX-1, -2 and -3) were the most commonly observed toxins found in the samples, indicating Pacific Ocean areas as the most important origin of ciguatera fish poisoning. Clinical diagnosis from ciguatera patients also revealed the predominance of neurological illnesses in most cases, supporting intoxication of Pacific origin. This study demonstrated the ability of laboratory analysis to identify and quantify Pacific CTXs in suspected fish samples, so as to support the clinical diagnosis of ciguatera. Comparative analysis (Student's t-test and Spearman's rank correlation analysis) on the two CTX detection methods showed approximate linearity for overall P-CTXs (P-CTX-1, -2 and -3)/P-CTX-1 alone as derived by LC-MS/MS and total toxicity levels (P-CTX-1 equivalent) as determined by MBA. The LC-MS/MS method coupled with the rapid extraction method could allow the detection of trace amount of CTXs at levels below the clinically relevant limit, 0.1 ppb P-CTX-1 in fish flesh. For practical application, the adoption of a two-tiered approach for testing, chemical analysis by LC-MS/MS for toxic fish screening, coupled with biological assay by MBA for final toxicity confirmation, was proposed for first-line screening of CTX in potentially contaminated fish samples in the market, with an aim to minimizing the use of laboratory mice and at the same time providing reasonably effective means for routine analysis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicon - Volume 86, August 2014, Pages 96–106
نویسندگان
, , ,