کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2065476 1076924 2008 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Venom of Collett's snake (Pseudechis colletti) blocks the binding of α-bungarotoxin to acetylcholine receptors at chick but not human neuromuscular junctions: A histochemical study
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
Venom of Collett's snake (Pseudechis colletti) blocks the binding of α-bungarotoxin to acetylcholine receptors at chick but not human neuromuscular junctions: A histochemical study
چکیده انگلیسی

Transverse cryosections, 6–8 μm thick, were cut from unfixed biventer cervicis muscles of chicks and quadriceps muscles of humans, mounted on glass slides and incubated for 1 h in either isotonic phosphate buffered saline, pH 7.3 (PBS), or crude venom of venom of Pseudechis colletti at concentrations between 2.1 and 210 μg ml−1 in PBS. They were then exposed to a fluoresceine-conjugated α-bungarotoxin to label ACh receptor sites. Exposure to the crude venom of P. colletti prevented the labelling of acetylcholine (ACh) receptors in chick muscle in a dose-dependant manner; at a concentration of 2.1 μg ml−1 labelling fell by 20% and at a concentration of 21 μg ml−1 by more than 90%. In contrast, exposure to the venom at concentrations as high as 210 μg ml−1 had no effect on receptor labelling in human skeletal muscle. The results suggest that ACh receptors in human skeletal muscle are relatively resistant to the postsynaptically active neurotoxins in the venom of P. colletti. The data explain the apparent anomaly that the venom blocks neuromuscular transmission in isolated nerve-muscle preparations of the chick whilst human subjects of envenoming bites by P. colletti exhibit no overt signs of neurotoxicity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicon - Volume 52, Issue 5, October 2008, Pages 647–650
نویسندگان
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