کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2065864 1076945 2008 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification and characterization of a new member of snake venom thrombin inhibitors from Bothrops insularis using a proteomic approach
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
Identification and characterization of a new member of snake venom thrombin inhibitors from Bothrops insularis using a proteomic approach
چکیده انگلیسی

Snake venom C-type lectin-like proteins (CLPs) are ubiquitously found in Viperidae snake venoms and differ from the C-type lectins as they display different biological activities but no carbohydrate-binding activity. Previous analysis of the transcriptome obtained from the Bothrops insularis venom gland showed the presence of two clusters homologous to bothrojaracin (BJC) chains α and β. In an effort to identify a new BJC-like molecule, we used an approach associated with proteomic technologies to identify the presence of the expressed protein and then to purify and characterize a new thrombin inhibitor from B. insularis venom. We also constructed homology models of this protein and BJC, which were compared with other C-type lectin-like family members and revealed several conserved features of this intriguing snake venom toxin family.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicon - Volume 51, Issue 4, 15 March 2008, Pages 659–671
نویسندگان
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