کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2065915 1076949 2008 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Vascular permeability and vasodilation induced by the Loxosceles intermedia venom in rats: Involvement of mast cell degranulation, histamine and 5-HT receptors
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
Vascular permeability and vasodilation induced by the Loxosceles intermedia venom in rats: Involvement of mast cell degranulation, histamine and 5-HT receptors
چکیده انگلیسی

The mechanisms involved in both local and systemic effects of Loxosceles intermedia (brown spider) venom (LIV) are still poorly understood. We show using rats treated with Evans blue dye (50 mg/kg, i.v.) that small doses of the LIV (0.1, 0.3, 1 and 3 μg/site) dose-dependently increase the vascular permeability in rats, an effect unchanged by indomethacin (5 mg/kg, i.p.), atropine (1 mg/kg, i.p.), HOE-140 (2 mg/kg, s.c.) or SR140333 (0.3 mg/kg, i.p.), but fully avoided by promethazine (15 mg/kg, i.p.), methysergide (2 mg/kg, i.p.) and compound 48/80 (3 mg/kg/day for 3 days). Addition of cumulative concentrations of LIV (0.1–5 μg) in phenylephrine-contracted aortic rings resulted in a partial (∼40%) and endothelium-dependent relaxation, inhibited by the nitric oxide synthase inhibitors l-NAME (10 μM) and l-NMMA (1 mM), and the guanylate cyclase inhibitors methylene blue (100 μM) and ODQ (10 μM). LIV-induced relaxation was abolished by compound 48/80 (10 μM) and pyrilamine (a selective histamine H1 receptor antagonist; 100 μM), but not by atropine (1 μM) and indomethacin (10 μM). Our results disclose that LIV increases vascular permeability and induces vascular relaxation. These effects occur due to its ability to degranulate mast cells and release mediators such as histamine and serotonine.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicon - Volume 51, Issue 3, 1 March 2008, Pages 363–372
نویسندگان
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