کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2091853 1545982 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Bacillus velezensis RC 218 as a biocontrol agent to reduce Fusarium head blight and deoxynivalenol accumulation: Genome sequencing and secondary metabolite cluster profiles
ترجمه فارسی عنوان
Bacillus velezensis RC 218 به عنوان عامل کنترل بیولوژیکی برای کاهش سرماخوردگی فوزاریوم و تجمع دگزینوالنول: توالی ژنوم و پروفایل های خوشه ای متابولیسم ثانویه
کلمات کلیدی
Bacillus velezensis؛ FHB biocontrol؛ توالی ژنوم؛ تولید متابولیت ثانویه؛ ericin Lantibiotic
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
چکیده انگلیسی

Bacillus subtilis RC 218 was originally isolated from wheat anthers as a potential antagonist of Fusarium graminearum, the causal agent of Fusarium head blight (FHB). It was demonstrated to have antagonist activity against the plant pathogen under in vitro and greenhouse assays. The current study extends characterizing B. subtilis RC 218 with a field study and genome sequencing. The field study demonstrated that B. subtilis RC 218 could reduce disease severity and the associated mycotoxin (deoxynivalenol) accumulation, under field conditions. The genome sequencing allowed us to accurately determine the taxonomy of the strain using a phylogenomic approach, which places it in the Bacillus velezensis clade. In addition, the draft genome allowed us to use bioinformatics to mine the genome for potential metabolites. The genome mining allowed us to identify 9 active secondary metabolites conserved by all B. velezensis strains and one additional secondary metabolite, the lantibiotic ericin, which is unique to this strain. This study represents the first confirmed production of ericin by a B. velezensis strain. The genome also allowed us to do a comparative genomics with its closest relatives and compare the secondary metabolite production of the publically available B. velezensis genomes. The results showed that the diversity in secondary metabolites of strains in the B. velezensis clade is driven by strains making different antibacterials.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbiological Research - Volume 192, November 2016, Pages 30–36
نویسندگان
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