کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2093918 1081985 2015 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Reprogramming of mouse amniotic fluid cells using a PiggyBac transposon system
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Reprogramming of mouse amniotic fluid cells using a PiggyBac transposon system
چکیده انگلیسی

Induced pluripotent stem (iPS) cells are generated from mouse and human somatic cells by forced expression of defined transcription factors using different methods. Amniotic fluid (AF) cells are easy to obtain from routinely scheduled procedures for prenatal diagnosis and iPS cells have been generated from human AF. Here, we generated iPS cells from mouse AF cells, using a non-viral-based approach constituted by the PiggyBac (PB) transposon system. All iPS cell lines obtained exhibited characteristics of pluripotent cells, including the ability to differentiate toward derivatives of all three germ layers in vitro and in vivo.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Stem Cell Research - Volume 15, Issue 3, November 2015, Pages 510–513
نویسندگان
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