کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2094140 1081991 2015 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The crucial role of vitamin C and its transporter (SVCT2) in bone marrow stromal cell autophagy and apoptosis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
The crucial role of vitamin C and its transporter (SVCT2) in bone marrow stromal cell autophagy and apoptosis
چکیده انگلیسی


• Oxidative stress induces autophagy in BMSCs.
• Vitamin C rescued the BMSCs from oxidative stress by regulating autophagy markers.
• Knockdown of the SVCT2 transporter in BMSCs dramatically decreased cell survival.
• SVCT2 knockdown BMSCs showed compromised autophagic flux.
• SVCT2 plays a vital role in the BMSC survival under stress conditions.

Vitamin C is an antioxidant that plays a vital role in various biological processes including bone formation. Previously, we reported that vitamin C is transported into bone marrow stromal cells (BMSCs) through the sodium dependent Vitamin C Transporter 2 (SVCT2) and this transporter plays an important role in osteogenic differentiation. Furthermore, this transporter is regulated by oxidative stress. To date, however, the exact role of vitamin C and its transporter (SVCT2) in ROS regulated autophagy and apoptosis in BMSCs is poorly understood. In the present study, we observed that oxidative stress decreased survival of BMSCs in a dose-dependent manner and induced growth arrest in the G1 phase of the cell cycle. These effects were accompanied by the induction of autophagy, confirmed by P62 and LC3B protein level and punctate GFP-LC3B distribution. The supplementation of vitamin C significantly rescued the BMSCs from oxidative stress by regulating autophagy. Knockdown of the SVCT2 transporter in BMSCs synergistically decreased cell survival even under low oxidative stress conditions. Also, supplementing vitamin C failed to rescue cells from stress. Our results reveal that the SVCT2 transporter plays a vital role in the mechanism of BMSC survival under stress conditions. Altogether, this study has given new insight into the role of the SVCT2 transporter in oxidative stress related autophagy and apoptosis in BMSCs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Stem Cell Research - Volume 15, Issue 2, September 2015, Pages 312–321
نویسندگان
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