کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2094399 | 1082014 | 2009 | 12 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Long-term microcarrier suspension cultures of human embryonic stem cells Long-term microcarrier suspension cultures of human embryonic stem cells](/preview/png/2094399.png)
The conventional method of culturing human embryonic stem cells (hESC) is on two-dimensional (2D) surfaces, which is not amenable for scale up to therapeutic quantities in bioreactors. We have developed a facile and robust method for maintaining undifferentiated hESC in three-dimensional (3D) suspension cultures on matrigel-coated microcarriers achieving 2- to 4-fold higher cell densities than those in 2D colony cultures. Stable, continuous propagation of two hESC lines on microcarriers has been demonstrated in conditioned media for 6 months. Microcarrier cultures (MC) were also demonstrated in two serum-free defined media (StemPro and mTeSR1). MC achieved even higher cell concentrations in suspension spinner flasks, thus opening the prospect of propagation in controlled bioreactors.
Journal: Stem Cell Research - Volume 2, Issue 3, May 2009, Pages 219–230