کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2105581 | 1546344 | 2009 | 10 صفحه PDF | دانلود رایگان |
Cytomegalovirus (CMV) reactivation was analyzed in 92 recipients of allogeneic hematopoietic stem cell transplantation (HSCT) in relation to the proportion of CD4+ lymphocytes in blood and a microsatellite polymorphism within the first intron of the interferon-gamma (IFNG) gene. CMV reactivation was found in 50% of the HSCT recipients; in 30% of these individuals, the level of CMV copies exceeded 100 per 105 peripheral blood (PB) cells on at least one occasion during the 100-day post-HSCT observation period. This high CMV copy level was most frequently found between 31 and 60 days post-HSCT (P = .021). Patients with ≥ 100 CMV copies/105 cells were characterized by poorer overall survival (OS) compared with those lacking CMV copies or having < 100 CMV copies/105 cells (P = .04), and they suffered from severe post-HSCT complications, including acute graft-versus-host disease (aGVHD) and relapse. Thus, patients with ≥ 100 CMV copies/105 cells were designated as having clinically significant CMV reactivation. Patients with < 10% CD4+ lymphocytes had a higher number of CMV DNA copies than those with higher proportions of CD4+ lymphocytes (0.62 vs 0.21, P = .001; mean ± SEM, 4422 ± 1667 vs 937 ± 662 CMV copies/105 cells, P < .001, for the proportion of cases with reactivation and numbers of copies, respectively). Similarly, patients carrying 2 IFNG 13-CA-repeat alleles (homozygotes) had more frequent CMV reactivation (0.50 vs 0.26; P = .039) and a higher CMV load (4111 ± 1699 vs 950±591 CMV copies/105 cells; P = .041) compared with those with other IFNG microsatellite allele constellations. Multivariate analysis demonstrated that the IFNG 13-CA-repeat homozygous genotype (odds ratio [OR] = 0.221; P = .044), a low proportion of CD4+ lymphocytes (OR = 0.276; P = .050), and a lack of optimal (10/10 alleles) donor–recipient HLA match (OR = 15.19; P = .006) were independent risk factors for CMV reactivation with a high number of copies.
Journal: - Volume 15, Issue 10, October 2009, Pages 1296–1305