کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2137171 1087834 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Isolation of siRNA target by biotinylated siRNA reveals that human CCDC12 promotes early erythroid differentiation
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Isolation of siRNA target by biotinylated siRNA reveals that human CCDC12 promotes early erythroid differentiation
چکیده انگلیسی
Erythroid differentiation is a tightly regulated multi-step process that has not been fully elucidated. We previously reported that a siRNA screened from random siRNA library, siRNA clone-67, induced erythroid differentiation in human erythroleukemia K-562 cell line. Here we identified that human CCDC12 (coiled-coil domain containing 12) is a target of siRNA clone-67, by target capture with biotinylated siRNA. Over-expression of CCDC12 in K-562 cell up-regulated the expression of CD235, ɛ-globin and γ-globin, accelerated cell growth, and slightly down-regulated the expression of GATA-2. Knockdown of CCDC12 slowed down the cell growth. These data indicate that CCDC12 is a new participant that promotes early erythroid differentiation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Leukemia Research - Volume 36, Issue 6, June 2012, Pages 779-783
نویسندگان
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