Peripheral blood and bone marrow TNF and TNF receptors in early and advanced stages of B-CLL in correlation with ZAP-70 protein and CD38 antigen

The aim of the study was to evaluate TNF and TNF receptor levels in peripheral blood (PB) and bone marrow (BM) in correlation with clinical and laboratory parameters of B-CLL patients. TNF concentration was significantly higher in BM than in PB. In B-CLL patients elevated TNF and sTNF-RII plasma levels had a high correlation with adverse prognostic factors such as ZAP-70 and CD38. Moreover, the percentage of TNF-producing T cells was significantly higher in ZAP-70-positive comparing to ZAP-70-negative patients. TNF and sTNF-RII plasma levels were higher in the group of patients requiring treatment comparing to the patients who did not.