کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2146530 1548354 2012 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Genome-wide analysis of mutagenesis bias and context sensitivity of N-methyl-N′-nitro-N-nitrosoguanidine (NTG)
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Genome-wide analysis of mutagenesis bias and context sensitivity of N-methyl-N′-nitro-N-nitrosoguanidine (NTG)
چکیده انگلیسی

We have analyzed the mutation spectrum of N-methyl-N′-nitro-N-nitrosoguanidine (NTG) from a set of 4099 mutations identified from whole-genome sequencing of 32 E. coli strains mutagenized with NTG. These data permit precise measurement of NTG's bias for G/C to A/T transitions (96.6% of all mutations) and also show that NTG mutagenesis is strongly sensitive to context, favoring guanine residues preceded by purines by five-fold over those preceded by pyrimidines. These data give confident estimates for the GC bias and transition/transversion ratios of NTG mutagenesis, which could not be estimated confidently from previous, much smaller datasets.


► Larger data set permits a genome-wide estimate of mutation spectrum due to NTG-induced mutagenesis.
► Smaller confidence intervals for the GC bias and transition/transversion ratios of NTG mutagenesis are given.
► NTG mutagenesis is shown to be sensitive to nucleotide context.
► Context sensitivity is measured in terms of conditional probabilities, which are not affected by variations in GC content.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis - Volume 731, Issues 1–2, 1 March 2012, Pages 64–67
نویسندگان
, ,