کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2146856 1548376 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A quantitative analysis of genomic instability in lymphoid and plasma cell neoplasms based on the PIG-A gene
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
A quantitative analysis of genomic instability in lymphoid and plasma cell neoplasms based on the PIG-A gene
چکیده انگلیسی

It has been proposed that hypermutability is necessary to account for the high frequency of mutations in cancer. However, historically, the mutation rate (μ) has been difficult to measure directly, and increased cell turnover or selection could provide an alternative explanation. We recently developed an assay for μ using PIG-A as a sentinel gene and estimated that its average value is 10.6 × 10−7 mutations per cell division in B-lymphoblastoid cell lines (BLCLs) from normal donors. Here we have measured μ in human malignancies and found that it was elevated in cell lines derived from T cell acute lymphoblastic leukemia, mantle cell lymphoma, follicular lymphoma in transformed phase, and 2 plasma cell neoplasms. In contrast, μ was much lower in a marginal zone lymphoma cell line and 5 other plasma cell neoplasms. The highest μ value that we measured, 3286 × 10−7, is 2 orders of magnitude above the range we have observed in non-malignant human cells. We conclude that the type of genomic instability detected in this assay is a common but not universal feature of hematologic malignancies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis - Volume 686, Issues 1–2, 1 April 2010, Pages 1–8
نویسندگان
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